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Browsing by Author "Abskharon, Romany N N"

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Combining in-situ proteolysis and microseed matrix screening to promote crystallization of PrPc-nanobody complexes

Abskharon, Romany N N; Soror, Sameh H; Pardon, Els; El Hassan, Hassan; Legname, Giuseppe; Steyaert, Jan; Wohlkonig, Alexandre (Oxford University Press, 2011-09)

Prion proteins (PrPs) are difficult to crystallize, probably due to their inherent flexibility. Several PrPs structures have been solved by nuclear magnetic resonance (NMR) techniques; however, only three structures were ...
Crystallization and preliminary X-ray diffraction analysis of a specific VHH domain against mouse prion protein.

Abskharon, Romany N N; Soror, Sameh H; Pardon, Els; El Hassan, Hassan; Legname, Giuseppe; Steyaert, Jan; Wohlkonig, Alexandre (Wiley, 2010-12)

Prion disorders are infectious diseases that are characterized by the conversion of the cellular prion protein PrPC into the pathogenic isoform PrPSc. Specific antibodies that interact with the cellular prion protein have ...
A novel expression system for production of soluble prion proteins in E. coli

Abskharon, Romany N N; Ramboarina, Stephanie; El Hassan, Hassan; Gad, Wael; Apostol, Marcin I; Giachin, Gabriele; Legname, Giuseppe; Steyaert, Jan; Messens, Joris; Soror, Sameh H; Wohlkonig, Alexandre (BioMed Central, 2012-01-10)

Expression of eukaryotic proteins in Escherichia coli is challenging, especially when they contain disulfide bonds. Since the discovery of the prion protein (PrP) and its role in transmissible spongiform encephalopathies, ...

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